Review



criterion tgx stain  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Bio-Rad criterion tgx stain
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criterion Tgx Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/10%25+Criterion+TGX+Stain-Free+Protein+Gel/pmc12828604-94-8-12
    Average 96 stars, based on 399 article reviews
    criterion tgx stain - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals"

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    Journal: Journal of Sport and Health Science

    doi: 10.1016/j.jshs.2025.101111

    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Figure Legend Snippet: Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Techniques Used: Staining, Membrane

    HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Figure Legend Snippet: HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Techniques Used: Staining, Membrane, Comparison

    Related Articles

    SDS Page:

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. 1c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Staining:

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. 1c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties
    Article Snippet: Samples were separated by SDS‐PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion TGX Stain‐Free Protein Gels (1656001, Bio‐Rad) and 1x SDS‐page running buffer (192 m m glycine, 3.5 m m SDS, 25 m m Tris‐hydroxymethyl‐methylamine). .. Following protein separation, the 4–20% Criterion TGX Stain‐Free Protein Gel (M3148, Bio‐Rad) was activated by a GelDoc XR+ (BioRad). .. Protein samples were transferred onto a 0.45 μm pore polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore), previously activated in cold 100% methanol, using a Criterion blotter (1704070, Bio‐Rad) at 100 V in transfer buffer (192 m m glycine, 20% v/v methanol, 25 m m tris‐hydroxymethyl‐methylamine) for 1.5 h. Following transfer, the membranes were washed in 0.05% Tween (P1379, Sigma) in PBS (PBST) on a rocker and imaged using GelDoc XR+ for total protein.

    Article Title: Lipophilic statins deplete GPX4 to promote ferroptosis and sensitize cancer cells to immune checkpoint blockade
    Article Snippet: 15 μg total protein was mixed with sample buffer (Thermo Fisher, B0007) and denatured at 95°C for 10 minutes. .. Sample was separated by Criterion TGX Stain-Free Protein Gel (Bio-Rad, #5678085), and transferred to a Nitrocellulose membrane (Bio-Rad, #P1620112). .. Membranes were blocked with 5% w/v nonfat dry milk and incubated with primary antibodies overnight at 4°C and HRP-conjugated secondary antibodies (Cell Signaling Technology (CST) Danvers, MA) for 1 hour at room temperature.

    Article Title: Electrode Reduction by Vibrio natriegens Depends on Balanced Expression of Multiheme Cytochromes
    Article Snippet: .. Thirty microliters of cell lysate was loaded into each well of a 4-20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678094) that was run at 300 V in 1X Tris/Glycine/SDS running buffer (Bio-Rad, 1610732) until the lowest mass band of the Precision Plus Protein Dual Color Standards ladder (Bio-Rad, 1610374) reached the bottom of the gel. .. The separated protein bands were transferred from the gel to a nitrocellulose membrane (Bio-Rad, 1704159) using a Trans-Blot Turbo Transfer System (Bio-Rad).

    Article Title: Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1A
    Article Snippet: Total protein was quantified using a DC protein assay kit (Bio-Rad: 5000111). .. For western blot analysis, 20 μg of protein per sample was loaded onto a 4%–20% Criterion TGX stain-free protein gel (Bio-Rad: 5678093) and transferred to a PVDF membrane (Bio-Rad: 1704275) using a semi-dry transfer system (Bio-Rad: 1704150). .. Membranes were blocked with 5% milk in tris-buffered saline with Tween 20 (TBS-T; 200 mM Tris, 1,500 mM NaCl, pH 7.6) for 1 h at room temperature, then incubated with a mouse anti-PMP22 antibody (1:500; Abcam: ab90782) overnight at 4°C.

    Membrane:

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. 1c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Lipophilic statins deplete GPX4 to promote ferroptosis and sensitize cancer cells to immune checkpoint blockade
    Article Snippet: 15 μg total protein was mixed with sample buffer (Thermo Fisher, B0007) and denatured at 95°C for 10 minutes. .. Sample was separated by Criterion TGX Stain-Free Protein Gel (Bio-Rad, #5678085), and transferred to a Nitrocellulose membrane (Bio-Rad, #P1620112). .. Membranes were blocked with 5% w/v nonfat dry milk and incubated with primary antibodies overnight at 4°C and HRP-conjugated secondary antibodies (Cell Signaling Technology (CST) Danvers, MA) for 1 hour at room temperature.

    Article Title: Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1A
    Article Snippet: Total protein was quantified using a DC protein assay kit (Bio-Rad: 5000111). .. For western blot analysis, 20 μg of protein per sample was loaded onto a 4%–20% Criterion TGX stain-free protein gel (Bio-Rad: 5678093) and transferred to a PVDF membrane (Bio-Rad: 1704275) using a semi-dry transfer system (Bio-Rad: 1704150). .. Membranes were blocked with 5% milk in tris-buffered saline with Tween 20 (TBS-T; 200 mM Tris, 1,500 mM NaCl, pH 7.6) for 1 h at room temperature, then incubated with a mouse anti-PMP22 antibody (1:500; Abcam: ab90782) overnight at 4°C.

    Western Blot:

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.
    Article Snippet: As a secondary measure of genetic confirmation and to ensure our genetic manipulations did not introduce undesirable modifications anywhere within the > 2 Mbp genome, genomic DNA was prepared (Monarch Genomic DNA Purification Kit, NEB, #T3010L) or cell pellets were stabilized (DNA/RNA Shield, Zymo Research, R1100) and genomic DNA was prepared (Plasmidsaurus) and the whole genome of every strain was sequenced via in-house or commercial Oxford Nanopore Technology sequencing at minimally ~ 30X coverage and often > 1000X coverage. .. Treated TCL was separated via SDS-PAGE on a 12% Criterion TGX Stain-Free Protein Gel (BioRad, 5678044) at 150 V for 55 min before being transferred to 0.2 μm polyvinylidene fluoride (PVDF) membrane and visualized via Western blotting employing 1:10,000 anti-HA 1 ̊ antibody (Invitrogen, 26183-1MG) made in mouse which binds to the HA epitope encoded on tethered proteins and a 1:1000 anti-mouse conjugated alkaline phosphatase 2 ̊ antibody (LGC Clinical Diagnostics, 5220-0312) that was used for colorimetric detection (Fig. 1c). .. Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1A
    Article Snippet: Total protein was quantified using a DC protein assay kit (Bio-Rad: 5000111). .. For western blot analysis, 20 μg of protein per sample was loaded onto a 4%–20% Criterion TGX stain-free protein gel (Bio-Rad: 5678093) and transferred to a PVDF membrane (Bio-Rad: 1704275) using a semi-dry transfer system (Bio-Rad: 1704150). .. Membranes were blocked with 5% milk in tris-buffered saline with Tween 20 (TBS-T; 200 mM Tris, 1,500 mM NaCl, pH 7.6) for 1 h at room temperature, then incubated with a mouse anti-PMP22 antibody (1:500; Abcam: ab90782) overnight at 4°C.

    other:

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.
    Article Snippet: Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).

    Article Title: Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis
    Article Snippet: Total cell lysate (TCL) from ~ 100 mls of culture grown to late-exponential phase was pelleted and resuspended in 25 mM Tris HCl pH 8.0, 500 mM NaCl, and 10% glycerol before being sonicated on ice for 10 s every 40 s for 5 min at 50% power (VibraCell, Sonics and Materials Inc.).



    Similar Products

    96
    Bio-Rad criterion tgx stain
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criterion Tgx Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/10%25+Criterion+TGX+Stain-Free+Protein+Gel/pmc12828604-94-8-12
    Average 96 stars, based on 1 article reviews
    criterion tgx stain - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad criterion tgx stain free protein gel
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criterion Tgx Stain Free Protein Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9320%25+Criterion+TGX+Stain-Free+Protein+Gel/pmc13051718-444-15-20
    Average 96 stars, based on 1 article reviews
    criterion tgx stain free protein gel - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad criterion tgx stain free precast gel
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criterion Tgx Stain Free Precast Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9315%25+Criterion+TGX+Stain-Free+Protein+Gel/bio_rxiv__64898__2026__04__30__722014-179-6-11
    Average 96 stars, based on 1 article reviews
    criterion tgx stain free precast gel - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad tgx stain free precast gel
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Tgx Stain Free Precast Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9320%25+Criterion+TGX+Stain-Free+Protein+Gel/bio_rxiv__64898__2026__04__30__721890-77-12-16
    Average 96 stars, based on 1 article reviews
    tgx stain free precast gel - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad criteriontm tgx stain freetm precast gel
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criteriontm Tgx Stain Freetm Precast Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9320%25+Criterion+TGX+Stain-Free+Protein+Gel/pmc13142990-115-8-16
    Average 96 stars, based on 1 article reviews
    criteriontm tgx stain freetm precast gel - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    92
    Bio-Rad polyacrylamide criteriontm tgx stain freetm precast gels
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Polyacrylamide Criteriontm Tgx Stain Freetm Precast Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/10%E2%80%9320%25+Criterion+TGX+Stain-Free+Protein+Gel/pmc13109771-89-11-17
    Average 92 stars, based on 1 article reviews
    polyacrylamide criteriontm tgx stain freetm precast gels - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    96
    Bio-Rad tgx protein gel
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Tgx Protein Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9320%25+Criterion+TGX+Stain-Free+Protein+Gel/pm42020607-90-13-16
    Average 96 stars, based on 1 article reviews
    tgx protein gel - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad criterion gels
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criterion Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9315%25+Criterion+TGX+Stain-Free+Protein+Gel/bio_rxiv__64898__2026__04__10__717554-322-26-28
    Average 96 stars, based on 1 article reviews
    criterion gels - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad criterion tgx stain free protein gels
    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see <t>Methods).</t> <t>Stain-free</t> gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
    Criterion Tgx Stain Free Protein Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+stain+free+protein+gel/4%E2%80%9315%25+Criterion+TGX+Stain-Free+Protein+Gel/10__1113_slash_jp290732-133-9-14
    Average 96 stars, based on 1 article reviews
    criterion tgx stain free protein gels - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Total protein was separated on 10% or 4%–15% Criterion TGX stain-free gels (Bio-Rad Laboratories) and run for 45 min at 200 V. Using a wet transfer protocol, protein was transferred to nitrocellulose membranes at 100 V for 30 min. Membranes were incubated in Miser TM solution (ThermoFisher Scientific) and blocked in 5% skim milk powder in tris-buffered saline-tween (TBST).

    Techniques: Staining, Membrane

    HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Total protein was separated on 10% or 4%–15% Criterion TGX stain-free gels (Bio-Rad Laboratories) and run for 45 min at 200 V. Using a wet transfer protocol, protein was transferred to nitrocellulose membranes at 100 V for 30 min. Membranes were incubated in Miser TM solution (ThermoFisher Scientific) and blocked in 5% skim milk powder in tris-buffered saline-tween (TBST).

    Techniques: Staining, Membrane, Comparison